detection of human bloodstains by enzyme-linked immunosorbent assay (elisa)

the microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (elisa) , usi ng alkaline phosphatase anti-human i gg conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed fr om human and laborat or y anima l s i n t he form of small dr ied b loodst a i ns on f ilt e r paper . the modi f i e d elisa t e chnique wa s compa red wit h t he agar double d i f f us ion me t hod of prec ipitin test . the results of this primary study showed t ha t the elisa i n compare to gel diffusi o n t e st is sensitive and specific. it is also enough repr oducible and practical t o be consider as a competent technique for i de nt i f i c ation o f human bloodstains in medicolegal laboratories .